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Year : 2013  |  Volume : 2  |  Issue : 1  |  Page : 27-31

Expression of CD7, CD13, CD14, CD33 and CD34 on Myeloblasts in Bone Marrow Aspirate of Patients with Newly Diagnosed Acute Myeloid Leukemia Using Multicolor Flow Cytometry

1 Al-Yarmouk Teaching Hospital, Department of Teaching Laboratories
2 Al- Nahrain University, College of Medicine, Department of Pathology

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Source of Support: None, Conflict of Interest: None

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Background: An important part of diagnosis of acute leukemia is to decide on the lineage whether it is of myeloid or lymphoid. Familiarity with expression of various surface antigen markers is essential to diagnose difficult cases and to follow up for minimum residual disease. Aim of the study: To evaluate the expression of CD7, CD13, CD14, CD33 and CD34 in bone marrow aspirate of patients with newly diagnosed AML using multicolor multiparametric flow cytometry. Patients, materials and methods: This is a prospective study which includes 21 newly diagnosed adult patients with AML from April 2012 to March 2013. Flow cytometry analysis for CD7, CD13, CD14, CD33 and CD34 was carried out on bone marrow aspirate samples using 2-laser, 4-color PARTEC Cube6 and using De Novo FCS Express 4 Flow Cytometry software. The sensitivity of fluorescent detectors was monitored using standard beads according to the manufacturer's recommendations and normal lymphoid cells within specimens served as internal positive and negative controls. Only samples with blasts that contain Auer rods, positive for SBB cytochemical stain (≥3%) and/or MPO+ by FC (≥10%) are included. Results: The median, range and interquartile range percentages for myeloblasts, CD7, CD13, CD14, CD33 and CD34 are 48, 22-84 and 24; 30, 20-36 and 7; 62, 34-98 and 28; 53, 21-86 and 6; 56, 30-91 and 17; and 73, 25-94 and 34respectively. Conclusions: The use of FC at diagnosis of AML can be useful to add objective confirmation especially in cases where Auer rods are not present and when SBB stain did not add towards the diagnosis. Moreover, if the aim is to follow up for MRD then the use of FC becomes essential.

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