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Year : 2014  |  Volume : 3  |  Issue : 2  |  Page : 116-126

Screening for BCR-ABL fusion gene among Iraqi children with ALL using real-time PCR

1 Department of Laboratory, Baghdad Teaching Hospital, Medical city center, Baghdad, Iraq
2 Department of Pathology, College of Medicine, Al-Nahrain University, Baghdad, Iraq
3 Department of Pediatrics, College of Medicine, Baghdad University, Baghdad, Iraq

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Source of Support: None, Conflict of Interest: None

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Background: Pediatric acute lymphoblastic leukemia (ALL) is a heterogeneous disease with diverse genetic subtypes and different clinical behavior. ALL with BCR-ABL is a subtype with distinctive response to treatment. Objectives: This study aims to study the expression of BCR-ABL transcript in Iraqi pediatric ALL patients. Patients, materials and methods: A case-control prospective study was conducted to study the expression of minor and major BCR-ABL fusion genes in bone marrow aspirates of 48 pre-treated ALL patients and 46 control subjects using qPCR technique. Those aspirates were collected from Children Welfare Teaching Hospital in Baghdad over a 1 year period from July 2013 to June 2014. Results: From 48 patients with ALL 21 were males and 27 were females. Age ranged from 2 months to 13 years with a median age of 5 years. Twenty six of these patients aged between 1-5 years. Molecular screening detected minor BCR-ABL transcript in 2.1% of ALL patients whereas major BCR-ABL transcript was not expressed in any of ALL patients. Contrary to the control group, minor BCR-ABL transcript was detected in three samples. Conclusion: The molecular prevalence of minor and major BCR-ABL fusion gene in Iraqi children with ALL is to somewhat similar to previous reports worldwide.

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